Double-flow focused liquid injector for efficient serial femtosecond crystallography
نویسندگان
چکیده
Serial femtosecond crystallography requires reliable and efficient delivery of fresh crystals across the beam of an X-ray free-electron laser over the course of an experiment. We introduce a double-flow focusing nozzle to meet this challenge, with significantly reduced sample consumption, while improving jet stability over previous generations of nozzles. We demonstrate its use to determine the first room-temperature structure of RNA polymerase II at high resolution, revealing new structural details. Moreover, the double-flow focusing nozzles were successfully tested with three other protein samples and the first room temperature structure of an extradiol ring-cleaving dioxygenase was solved by utilizing the improved operation and characteristics of these devices [corrected].
منابع مشابه
Corrigendum: Double-flow focused liquid injector for efficient serial femtosecond crystallography
This corrects the article DOI: 10.1038/srep44628.
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عنوان ژورنال:
دوره 7 شماره
صفحات -
تاریخ انتشار 2017